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| Rachagani,Satyanarayana; Gupta,Ishwar Dayal. |
| Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism was detected by digestion of PCR-amplified products with HindIII, HhaI and HaeIII restriction enzymes, followed by separation on 3% agarose gels, and resolved by ethidium bromide staining. Allele A of the κ-casein gene occurred at a higher frequency than allele B, in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in the Sahiwal and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Indian cattle; Κ-casein; Genetic polymorphism; PCR-RFLP. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500015 |
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| Freitas,D.R.; Souza,F.N.; Fonseca,L.M.; Ladeira,C.V.G.; Santos,V.P.F.; Diniz,S.A.; Silva,M.X.; Haddad,J.P.A.; Cerqueira,M.M.O.P.. |
| ABSTRACT The present study attempted to identify individual milk proteins and other milk components that are associated with casein micelle size (CMS) and dry matter cheese yield (DMCY) using factor analysis. Here, we used 140 bulk tank milk samples from different farms. Milk composition was determined using a Fourier transform infrared equipament. The individual milk proteins were (αS-casein, β-casein, κ-casein, β-lactoglobulin and α-lactoalbumin) measured by their electrophoretic profile. The CMS was estimated by photon correlation spectroscopy, and the DMCY was determined using reduced laboratory-scale cheese production. Factor analysis partitioned the milk components into three groups that, taken together, explain 68.3% of the total variance. The first... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Κ-casein; Αs-casein; Β-casein; Α-lactalbumin; Β-lactoglobulin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501319 |
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